czapek medium meaning in Chinese
恰佩克培养基
Examples
- These vectors were tried to transform into the p . digitatum imazalil - sensitive isolate by the methods of peg - mediated or electroporation transformation . after that , the transformed protoplasts were regenerated on czapek medium containing hygromycin at 300 # g / ml . unfortunately , the ideal transformants could not be obtained
采用peg法和电击法两种方法进行指状青霉原生质体的转化，然后将得到的转化产物转移到查氏再生培养基中培养，遗憾的是没有得到理想的转化子。 - The effect of some factors , including the appropriate materials for isolating protoplasts , the concentrations of enzyme , period of digestion and temperatures , and osmotic pressure stabilizers on the isolation and regeneration of protoplasts in penicillium digitatum were studied . the results demonstrated that the purified protoplasts could regenerate through double layers of czapek medium containing 0 . 7mol / l nacl . the regeneration rate could reach 24 . 9 %
通过对制备材料、酶液浓度、酶解时间、酶解温度、渗透压稳定剂的种类和浓度等因素的实验研究，得到了一套制备指状青霉( penicilliumdigitatum )原生质体的有效方法，并在双层培养基上初步实现了原生质体的再生，再生率可达24 . 9 。